|The Haag-Streit lab could be built and equipped thanks to the generous donation from the Haag-Streit Group.|
A near infrared ultrashort pulse laser system consisting of a mode-locked oscillator (Coherent Mantis) and a chirped-pulse amplifier (Coherent RegA 9050 pumped with a Coherent Verdi V-12) produces laser pulses with a pulse length of about 60 fs and a pulse energy of around 6 uJ. The laser beam can be guided to various experimental setups on two vibration-isolated optical tables. The tables are placed under flow-boxes to ensure a dust-free, constant air flow.
One table is equipped with a Nikon A1-R MP microscope that allows confocal laser scanning microscopy and, thanks to a second mode-locked oscillator (Coherent Chameleon Vision II), multiphoton microscopy. The fluorescence can be detected either by high quantum yield 4-channel detector block, a 32-channel spectral detector block or a 4-channel non-descanned detector block. The aforementioned amplified laser beam is coupled into this microscope, which allows simultaneous optical visualization and sub-micrometer manipulation of a sample. Additionally this microscope has a single-photon counting camera attached (Andor XIon) to efficiently measure epifluorescence.
The other table is equipped with an atomic force microscope (Veeco Bioscope II) built on top of a Zeiss Axiovert 200. This type of AFM allows simultaneous sub-nanometer topographical scans and conventional optical microscopy. The amplified laser is coupled into this microscope as well, which again allows manipulation and simultaneous visualization (either using AFM or optical microscopy) of a sample. This microscope is additionally equipped with a time correlated single photon counting unit (TauMap, Jenlab) to measure fluorescence and luminescence decay times.
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|Femtosecond lasers in fluid inclusions laboratory|
|The laboratory is equipped with a near infrared ultrashort pulse laser system consisting of a mode-locked oscillator (Coherent Mira 900) and a chirped-pulse amplifier (Coherent RegA 9000 pumped with a Coherent Verdi V-10) produces laser pulses with a pulse length of about 160 fs and a pulse energy of around 4 uJ. The laser beam can be guided to various experimental setups on two optical tables.|
The beam is coupled into two inverse Olympus BX51 microscopes, both equipped with heating-cooling stages for fluid inclusion analysis. One stage was bought from Linkam, the other was custom built in our in-house workshop to address our need to investigate whole thin sections of minerals instead of just small fragments. The laser system can be used simultaneously on both systems thanks to a custom built beam path switcher. Each microscope has a highly sensitive camera attached (pco.1600 and pco.sensicam).
On the second table, the laser beam is coupled into a custom built multiphoton microscope, consisting of a galvanic mirror pair (GSI Lumonics) and a Zeiss Axiovert 100TV. This microscope is also equipped with a piezo stage (PI) which allows additional sample scanning.
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|Laser tissue soldering and electrospinning laboratory|
|The laboratory is equipped with all the apparatus that are needed for laser tissue soldering. It contains a compact diode laser Lina-30D (Intros Lasertechnology), a test stand for measuring the tensile strength and the liquid pressure of the tissue connection and a thermal camera A655sc (FLIR) to control the heat diffusion during soldering.|
Additionally the laboratory is appareled with a custom built electrospinning facility to produce patches for laser tissue soldering. Our setup is capable of spinning two separate polymers in parallel onto a rotating collector.
|>> Laser Tissue Soldering|